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KMID : 0361019950380111768
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Korean Journal of Otolaryngology - Head and Neck Surgery
1995 Volume.38 No. 11 p.1768 ~ p.1772
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Detection of Epstein Barr Virus RNA in Paraffin Section of Nasopharyngel Carcinoma Using in Situ Hybridization
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왕수건
이배혁/이동건/홍대영/김재혁/노환중/고의경/전경명/조군제
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Abstract
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To investigate the pathogenesis of nasopharyngeal carcinoma, we tried to detect Epstein-Barr virus(EBV) in nasopharyngeal carcinoma by in situ hybridization. The detection of EBV in neoplastic cells of nasopharyngeal carcinoma may be also useful
in
diagnosis in nonendemic area. Cellular localization of viral genome in tissue section can be usually identified by in situ hybridization. But demonstration of EBV nucleic acids in formalin-fixed paraffin-embedded tissue secions is difficult and
insensitive.
We performed rapid colorimetric in situ hybridization for detection of EBV RNA using a bioinylated oligoprobe. The EBER1 probe is a marker of latent EBV infection.
It was clearly demonstrated that EBV RNA is localized on neoplastic cells of undifferentiated carcinoma.
These results demonstrated that detection EBV RNA using in situ hybridization is very sensitive and reliable method and clearly shows the relationship between neoplastic cells infected with EBV and expession of EBV genome. (Korean J Otolaryngol
38:11,
1995)
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